Well it’s been a minute since I posted for the #atozlabchallenge
or otherwise…I’ve been pretty busy and also lost my motivation to some degree, but seeing how well this page has been received and how many people are interested in learning about and sharing my little corner of science has boosted me again
“Publish or perish” perhaps the most infamous adage of science, and indeed even academia. Publishing work is essentially how a scientist builds their reputation so publishing in top journals, such as the likes of Science and Nature, really can spotlight you and your work. In my line of work, Blood and BJH are big journals…what are the top journals in your area?
Reading a scientific article can be tough when you start, because of the concise writing style and technical language, but it soon becomes second nature and undoubtedly helps your own writing…so stick at it if you’re new! I hope you like my collage 😂
Mitochondrial health can be assessed to give an early indication of cellular apoptosis – ie ‘programmed cell death’ – JC-1 is a dye which facilitates this assessment. The positively charged dye binds to the negatively charged Electron Transport Chain of the inner mitochondria, where the dye aggregates and fluoresces red ➡️ when mitochondria integrity becomes compromised the negative charge is lost (depolarisation) and the dye diffuses throughout the cell where it fluoresces green instead 😮
The fluorescence can be captured by a plate reader, by flow cytometry, or by microscopy so it’s pretty versatile, and the fluorescent molecules give it this pretty colour 🤙
📸3 JUST KEEP GOING!//
Okay, so J is a pretty hard letter so here’s a message rather than a cool word or thing 🤫
Failing is integral to science. Fact. Here’s me analysing a multicolour flow experiment that has taken the best part of 8 months to crack, and the feeling of success was fairly euphoric. But to get to this point I had to tweak so many things so many times and observe the effects, using them to inform my next set of tweaks. That’s a pretty good summary for lab work, so if you're stuck just keep going!